It is crucial to study a wide range of samples before concluding variations amongst
countries, provenances or even a large population. It is also essential to compare
results of different extraction methods from the uniform seed lot. Results of extraction
techniques using different seed lots can lead to misleading results as there is a wide
variability among Neem trees and populations. Conditions that potential for
Azadirachtin A and B accumulation was influenced by environmental conditions could
not be drawn since individual trees of a particular agro-climatic zone showed different
trends.
Hasty conclusion of proposing a particular
country or provenance yielding high Azadirachtin
may be risky. Findings based on small sample
size or conclusions based on multitude of
factors can be misleading. With nearly 70
countries in the world interested to raise Neem
plantations, it is crucial that issues regarding
variability in Neem are understood. It can be
concluded from present study that there are
individual genetic differences among Neem
trees and synthesis of azadirachtins is not
dependent upon temperature, humidity or rainfall.
Genetic variability for Azadirachtin may affect the final products. Large variability
provides ample opportunity for selection and further improvement. It may not be
feasible at this stage to select a particular individual with desired traits as no
selections have been made in Neem so far. A systematic study for tree improvement
with a population of mother trees with desired traits should be undertaken by laying
half-sibprogeny trails and further selections by clonal propagations. Similarly multiple
location trials of identified germplasm are essential to resolve controversies and
develop site selected elite material. Selected and identified material should be
accessioned, documented and conserved for long to medium term storage by
various countries following Convention of Biodiversity norms and Farmers’ Rights
and Plant Varieties Protection Act of India. These acts will not only facilitate
commercialization, improvement of genetic stock but will also assist in benefit
sharing, awareness and acceptance of Neem as a potential industrial crop.
Pest and insects, disease causing pathogens, etc. are estimated to cause an annual loss of about 30-35% of the potential food world
wide. In India, according to a very conservative estimate, the annual losses of agricultural products due to pest infestation is about Rs.
25,000 crores. Although many strategies including Bt transgenic crops are being adopted the use of Neem in pest control is the most safe
and reliable method. Studies have revealed that the neem possesses about a dozen natural pest control properties and the Neem
products are ecologically sound and safe.
Studies on Neem